A guidance system is a virtual or physical device, or a group of devices implementing a controlling the movement of a ship, aircraft, missile, rocket, satellite, or any other moving object. Guidance is the process of calculating the changes in position, velocity, altitude, and/or rotation rates of a moving object required to follow a certain trajectory and/or altitude profile based on information about the object's state of motion.

A guidance system is usually part of a Guidance, navigation and control system, whereas navigation refers to the systems necessary to calculate the current position and orientation based on sensor data like those from compasses, GPS receivers, Loran-C, star trackers, inertial measurement units, altimeters, etc. The output of the navigation system, the navigation solution, is an input for the guidance system, among others like the environmental conditions (wind, water, temperature, etc.) and the vehicle's characteristics (i.e. mass, control system availability, control systems correlation to vector change, etc.). In general, the guidance system computes the instructions for the control system, which comprises the object's actuators (e.g., thrusters, reaction wheels, body flaps, etc.), which are able to manipulate the flight path and orientation of the object without direct or continuous human control.

One of the earliest examples of a true guidance system is that used in the German V-1 during World War II. The navigation system consisted of a simple gyroscope, an airspeed sensor, and an altimeter. The guidance instructions were target altitude, target velocity, cruise time, and engine cut off time.

A guidance system has three major sub-sections: Inputs, Processing, and Outputs. The input section includes sensors, course data, radio and satellite links, and other information sources. The processing section, composed of one or more CPUs, integrates this data and determines what actions, if any, are necessary to maintain or achieve a proper heading. This is then fed to the outputs which can directly affect the system's course. The outputs may control speed by interacting with devices such as turbines, and fuel pumps, or they may more directly alter course by actuating ailerons, rudders, or other devices.